Phosphorylation of plasma membrane H⁺-ATPase Thr881 participates in light-induced stomatal opening.

ABSTRACT: Plasma membrane (PM) H⁺-ATPase is crucial for light-induced stomatal opening and phosphorylation of a penultimate residue, Thr948 (pen-Thr, numbering according to Arabidopsis AHA1) is required for enzyme activation. In this study, a comprehensive phosphoproteomic analysis using guard cell protoplasts from Vicia faba shows that both red and blue light increase the phosphorylation of Thr881, of PM H⁺-ATPase. Light-induced stomatal opening and the blue light-induced increase in stomatal conductance are reduced in transgenic Arabidopsis plants expressing mutant AHA1-T881A in aha1-9, whereas the blue light-induced phosphorylation of pen-Thr is unaffected. Auxin and photosynthetically active radiation induce the phosphorylation of both Thr881 and pen-Thr in etiolated seedli

SUBMITTER: Hayashi Y 

PROVIDER: S-EPMC10879185 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature