Electrodeposited Gold on Carbon-Fiber Microelectrodes for Enhancing Amperometric Detection of Dopamine Release from Pheochromocytoma Cells.

ABSTRACT: Exocytosis is an ultrafast cellular process which facilitates neuron-neuron communication in the brain. Microelectrode electrochemistry has been an essential tool for measuring fast exocytosis events with high temporal resolution and high sensitivity. Due to carbon fiber's irreproducible and inhomogeneous surface conditions, however, it is often desirable to develop simple and reproducible modification schemes to enhance a microelectrode's analytical performance for single-cell analysis. Here we present carbon-fiber microelectrodes (CFEs) modified with a thin film of electrodeposited gold for the detection of exocytosis from rat pheochromocytoma cells (PC12), a model cell line for neurosecretion. These new probes are made by a novel voltage-pulsing deposition procedure and demonstrate impr

SUBMITTER: Barlow ST 

PROVIDER: S-EPMC10879420 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature