Multi-omics analysis on the mechanism of the effect of Isatis leaf on the growth performance of fattening sheep.

ABSTRACT:

Introduction

This study evaluated the effects of Isatis Leaf (ISL) on the growth performance, gastrointestinal tissue morphology, rumen and intestinal microbiota, rumen, serum and urine metabolites, and rumen epithelial tissue transcriptome of fattening sheep.

Methods

Twelve 3.5-month-old healthy fattening sheep were randomly divided into two groups, each with 6 replicates, and fed with basal diet (CON) and basal diet supplemented with 80 g/kg ISL for 2.5 months. Gastrointestinal tract was collected for histological analysis, rumen fluid and feces were subjected to metagenomic analysis, rumen fluid, serum, and urine for metabolomics analysis, and rumen epithelial tissue for transcriptomics analysis.

Results

The results showed that in the ISL group, the average daily g

SUBMITTER: Cao Z 

PROVIDER: S-EPMC10879442 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature