Single cell atlas of human gastric muscle immune cells and macrophage-driven changes in idiopathic gastroparesis.

ABSTRACT: Gastrointestinal immune cells, particularly muscularis macrophages (MM) interact with the enteric nervous system and influence gastrointestinal motility. Here we determine the human gastric muscle immunome and its changes in patients with idiopathic gastroparesis (IG). Single cell sequencing was performed on 26,000 CD45⁺ cells obtained from the gastric tissue of 20 subjects. We demonstrate 11 immune cell clusters with T cells being most abundant followed by myeloid cells. The proportions of cells belonging to the 11 clusters were similar between IG and controls. However, 9/11 clusters showed 578-11,429 differentially expressed genes. In IG, MM had decreased expression of tissue-protective and microglial genes and increased the expression of monocyte trafficking and stromal activ

SUBMITTER: Chikkamenahalli LL 

PROVIDER: S-EPMC10879712 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature