Radical generation and bactericidal activity of nanobubbles produced by ultrasonic irradiation of carbonated water.

ABSTRACT: Our previous study showed that nanobubbles (NBs) encapsulating CO₂ gas have bactericidal activity due to reactive oxygen species (ROS) (Yamaguchi et al., 2020). Here, we report that bulk NBs encapsulating CO₂ can be efficiently generated by ultrasonically irradiating carbonated water using a piezoelectric transducer with a frequency of 1.7 MHz. The generated NBs were less than 100 nm in size and had a lifetime of 500 h. Furthermore, generation of ROS in the NB suspension was investigated using electron spin resonance spectroscopy and fluorescence spectrometry. The main ROS was found to be the hydroxyl radical, which is consistent with our previous observations. The bactericidal activity lasted for at least one week. Furthermore, a mist generated by atomizing the NB su

SUBMITTER: Mokudai T 

PROVIDER: S-EPMC10879770 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature