Measuring Dynamic Glycosomal pH Changes in Living Trypanosoma brucei.

ABSTRACT: Glucose metabolism is critical for the African trypanosome, Trypanosoma brucei, as an essential metabolic process and regulator of parasite development. Little is known about the cellular responses generated when environmental glucose levels change. In both bloodstream and procyclic form (insect stage) parasites, glycosomes house most of glycolysis. These organelles are rapidly acidified in response to glucose deprivation, which likely results in the allosteric regulation of glycolytic enzymes such as hexokinase. In previous work, localizing the chemical probe used to make pH measurements was challenging, limiting its utility in other applications. This paper describes the development and use of parasites that express glycosomally localized pHluorin2, a heritable protein pH biosensor. pHlu

SUBMITTER: Call D 

PROVIDER: S-EPMC10879817 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature