DNA satellite and chromatin organization at mouse centromeres and pericentromeres.

ABSTRACT:

Background

Centromeres are essential for faithful chromosome segregation during mitosis and meiosis. However, the organization of satellite DNA and chromatin at mouse centromeres and pericentromeres is poorly understood due to the challenges of assembling repetitive genomic regions.

Results

Using recently available PacBio long-read sequencing data from the C57BL/6 strain, we find that contrary to the previous reports of their homogeneous nature, both centromeric minor satellites and pericentromeric major satellites exhibit a high degree of variation in sequence and organization within and between arrays. While most arrays are continuous, a significant fraction is interspersed with non-satellite sequences, including transposable elements. Using chromatin immunoprecipitation s

SUBMITTER: Packiaraj J 

PROVIDER: S-EPMC10880262 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature