Single-cell RNA sequencing of anaplastic ependymoma and H3K27M-mutant diffuse midline glioma.

ABSTRACT:

Background

Anaplastic ependymoma and H3K27M-mutant diffuse midline glioma are two common subtypes of brain tumors with poor long-term prognosis. The present study analyzed and compared the differences in cell types between two tumors by single-cell RNA sequencing (scRNA-seq) technology.

Methods

ScRNA-seq was performed to profile cells from cancer tissue from anaplastic ependymoma patient and H3K27M-mutant diffuse midline glioma patient. Cell clustering, marker gene identification, cell type annotation, copy number variation analysis and function analysis of differentially expressed genes were then performed.

Results

A total of 11,219 cells were obtained from anaplastic ependymoma and H3K27M mutant diffuse midline glioma, and these cells categorized into 12 distinct cl

SUBMITTER: Zang D 

PROVIDER: S-EPMC10880286 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature