The pathogenicity of vancomycin-resistant Enterococcus faecalis to colon cancer cells.

ABSTRACT:

Background

The aim of this study was to investigate the pathogenicity of vancomycin-resistant Enterococcus faecalis (VREs) to human colon cells in vitro.

Methods

Three E. faecalis isolates (2 VREs and E. faecalis ATCC 29212) were cocultured with NCM460, HT-29 and HCT116 cells. Changes in cell morphology and bacterial adhesion were assessed at different time points. Interleukin-8 (IL-8) and vascular endothelial growth factor A (VEGFA) expression were measured via RT-qPCR and enzyme-linked immunosorbent assay (ELISA), respectively. Cell migration and human umbilical vein endothelial cells (HUVECs) tube formation assays were used for angiogenesis studies. The activity of PI3K/AKT/mTOR signaling pathway was measured by Western blotting.

Results

The growth and adhesion of

SUBMITTER: Zhang L 

PROVIDER: S-EPMC10880345 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature