Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Substrates Promotes Neural Priming.

ABSTRACT: Electrical stimulation (ES) within a conductive scaffold is potentially beneficial in encouraging the differentiation of stem cells toward a neuronal phenotype. To improve stem cell-based regenerative therapies, it is essential to use electroconductive scaffolds with appropriate stiffnesses to regulate the amount and location of ES delivery. Herein, biodegradable electroconductive substrates with different stiffnesses are fabricated from chitosan-grafted-polyaniline (CS-g-PANI) copolymers. Human mesenchymal stem cells (hMSCs) cultured on soft conductive scaffolds show a morphological change with significant filopodial elongation after electrically stimulated culture along with upregulation of neuronal markers and downregulation of glial markers. Compared to stiff conductive scaffolds and n

SUBMITTER: Eftekhari BS 

PROVIDER: S-EPMC10880582 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature