Transcriptional heterogeneity of catabolic genes on the plasmid pCAR1 causes host-specific carbazole degradation.

ABSTRACT: To elucidate why plasmid-borne catabolic ability differs among host bacteria, we assessed the expression dynamics of the P_ant promoter on the carbazole-degradative conjugative plasmid pCAR1 in Pseudomonas putida KT2440(pCAR1) (hereafter, KTPC) and Pseudomonas resinovorans CA10. The P_ant promoter regulates the transcription of both the car and ant operons, which are responsible for converting carbazole into anthranilate and anthranilate into catechol, respectively. In the presence of anthranilate, transcription of the P_ant promoter is induced by the AraC/XylS family regulator AntR, encoded on pCAR1. A reporter cassette containing the P_ant promoter followed by gfp was inserted into the

SUBMITTER: Suzuki-Minakuchi C 

PROVIDER: S-EPMC10880608 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature