Identification of a novel growth-associated promoter for biphasic expression of heterogenous proteins in Pichia pastoris.

ABSTRACT: Pichia pastoris (P. pastoris) is one of the most popular cell factories for expressing exogenous proteins and producing useful chemicals. The alcohol oxidase 1 promoter (P_AOX1) is the most commonly used strong promoter in P. pastoris and has the characteristic of biphasic expression. However, the inducer for P_AOX1, methanol, has toxicity and poses risks in industrial settings. In the present study, analyzing transcriptomic data of cells collected at different stages of growth found that the formate dehydrogenase (FDH) gene ranked 4960th in relative expression among 5032 genes during the early logarithmic growth phase but rose to the 10th and 1st during the middle and late logarithmic growth phases, respectively, displaying a strict biphasic expres

SUBMITTER: Shen Q 

PROVIDER: S-EPMC10880622 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature