Single-Cell RNA-Seq Reveals Coronary Heterogeneity and Identifies CD133⁺TRPV4^high Endothelial Subpopulation in Regulating Flow-Induced Vascular Tone in Mice.

ABSTRACT:

Background

Single-cell RNA-Seq analysis can determine the heterogeneity of cells between different tissues at a single-cell level. Coronary artery endothelial cells (ECs) are important to coronary blood flow. However, little is known about the heterogeneity of coronary artery ECs, and cellular identity responses to flow. Identifying endothelial subpopulations will contribute to the precise localization of vascular endothelial subpopulations, thus enabling the precision of vascular injury treatment.

Methods

Here, we performed a single-cell RNA sequencing of 31 962 cells and functional assays of 3 branches of the coronary arteries (right coronary artery/circumflex left coronary artery/anterior descending left coronary artery) in wild-type mice.

Results

We found a compen

SUBMITTER: Mao A 

PROVIDER: S-EPMC10880935 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature