Regulation of YAP Promotor Accessibility in Endothelial Mechanotransduction.

ABSTRACT:

Background

Endothelial cells are constantly exposed to mechanical forces in the form of fluid shear stress, extracellular stiffness, and cyclic strain. The mechanoresponsive activity of YAP (Yes-associated protein) and its role in vascular development are well described; however, whether changes to transcription or epigenetic regulation of YAP are involved in these processes remains unanswered. Furthermore, how mechanical forces are transduced to the nucleus to drive transcriptional reprogramming in endothelial cells is poorly understood. The YAP target gene, AmotL2 (angiomotin-like 2), is a junctional mechanotransducer that connects cell-cell junctions to the nuclear membrane via the actin cytoskeleton.

Methods

We applied mechanical manipulations including shear flow

SUBMITTER: Mannion AJ 

PROVIDER: S-EPMC10880945 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature