Terpenoid balance in <i>Aspergillus nidulans</i> unveiled by heterologous squalene synthase expression.

ABSTRACT: Filamentous fungi produce numerous uncharacterized natural products (NPs) that are often challenging to characterize because of cryptic expression in laboratory conditions. Previously, we have successfully isolated novel NPs by expressing fungal artificial chromosomes (FACs) from a variety of fungal species into Aspergillus nidulans. Here, we demonstrate a twist to FAC utility wherein heterologous expression of a Pseudogymnoascus destructans FAC in A. nidulans altered endogenous terpene biosynthetic pathways. In contrast to wild type, the FAC transformant produced increased levels of squalene and aspernidine type compounds, including three new nidulenes (1- 2, and 5), and lost nearly all ability to synthesize the major A. nidulans characteri

SUBMITTER: Park SC 

PROVIDER: S-EPMC10881027 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature