From breast cancer cell homing to the onset of early bone metastasis: The role of bone (re)modeling in early lesion formation.

ABSTRACT: Breast cancer often metastasizes to bone, causing osteolytic lesions. Structural and biophysical changes are rarely studied yet are hypothesized to influence metastasis. We developed a mouse model of early bone metastasis and multimodal imaging to quantify cancer cell homing, bone (re)modeling, and onset of metastasis. Using tissue clearing and three-dimensional (3D) light sheet fluorescence microscopy, we located enhanced green fluorescent protein-positive cancer cells and small clusters in intact bones and quantified their size and spatial distribution. We detected early bone lesions using in vivo microcomputed tomography (microCT)-based time-lapse morphometry and revealed altered bone (re)modeling in the absence of detectable lesions. With a new microCT image analysis tool, we tracked t

SUBMITTER: Young SAE 

PROVIDER: S-EPMC10881061 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature