Pycofitness-Evaluating the fitness landscape of RNA and protein sequences.

ABSTRACT:

Motivation

The accurate prediction of how mutations change biophysical properties of proteins or RNA is a major goal in computational biology with tremendous impacts on protein design and genetic variant interpretation. Evolutionary approaches such as coevolution can help solving this issue.

Results

We present pycofitness, a standalone Python-based software package for the in silico mutagenesis of protein and RNA sequences. It is based on coevolution and, more specifically, on a popular inverse statistical approach, namely direct coupling analysis by pseudo-likelihood maximization. Its efficient implementation and user-friendly command line interface make it an easy-to-use tool even for researchers with no bioinformatics background. To illustrate its strengths, we present th

SUBMITTER: Pucci F 

PROVIDER: S-EPMC10881095 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature