Pycallingcards: an integrated environment for visualizing, analyzing, and interpreting Calling Cards data.

ABSTRACT:

Motivation

Unraveling the transcriptional programs that control how cells divide, differentiate, and respond to their environments requires a precise understanding of transcription factors' (TFs) DNA-binding activities. Calling cards (CC) technology uses transposons to capture transient TF binding events at one instant in time and then read them out at a later time. This methodology can also be used to simultaneously measure TF binding and mRNA expression from single-cell CC and to record and integrate TF binding events across time in any cell type of interest without the need for purification. Despite these advantages, there has been a lack of dedicated bioinformatics tools for the detailed analysis of CC data.

Results

We introduce Pycallingcards, a comprehensive Python mod

SUBMITTER: Guo J 

PROVIDER: S-EPMC10881108 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature