Influence of pump laser fluence on ultrafast myoglobin structural dynamics.

ABSTRACT: High-intensity femtosecond pulses from an X-ray free-electron laser enable pump-probe experiments for the investigation of electronic and nuclear changes during light-induced reactions. On timescales ranging from femtoseconds to milliseconds and for a variety of biological systems, time-resolved serial femtosecond crystallography (TR-SFX) has provided detailed structural data for light-induced isomerization, breakage or formation of chemical bonds and electron transfer^1,2. However, all ultrafast TR-SFX studies to date have employed such high pump laser energies that nominally several photons were absorbed per chromophore^3-17. As multiphoton absorption may force the protein response into non-physiological pathways, it is of great concern^18,19 whether this ex

SUBMITTER: Barends TRM 

PROVIDER: S-EPMC10881388 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature