Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons.

ABSTRACT: Basal forebrain cholinergic neurons (BFCN) participate in processes of learning, memory, and attention. Little is known about the genes expressed by BFCN and the extracellular signals that control their expression. Previous studies showed that bone morphogenetic protein (BMP) 9 induces and maintains the cholinergic phenotype of embryonic BFCN. We measured gene expression patterns in septal cultures of embryonic day 14 mice and rats grown in the presence or absence of BMP9 by using species-specific microarrays and validated the RNA expression data of selected genes by immunoblot and immunocytochemistry analysis of their protein products. BMP9 enhanced the expression of multiple genes in a time-dependent and, in most cases, reversible manner. The set of BMP9-responsive genes was concordant b

SUBMITTER: Lopez-Coviella I 

PROVIDER: S-EPMC1088172 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature