Vitamin B12 analogues from gut microbes and diet differentially impact commensal propionate producers of the human gut.

ABSTRACT: To produce the health-associated metabolite propionate, gut microbes require vitamin B12 as a cofactor to convert succinate to propionate. B12 is sourced in the human gut from the unabsorbed dietary fraction and in situ microbial production. However, experimental data for B12 production by gut microbes is scarce, especially on their produced B12-analogues. Further, the promotion of propionate production by microbially-produced and dietary B12 is not yet fully understood. Here, we demonstrated B12 production in 6 out of 8 in silico predicted B12-producing bacteria from the human gut. Next, we showed in vitro that B12 produced by Blautia hydrogenotrophica, Marvinbryantia formatexigens, and Blautia producta promoted succinate to propionate conversion

SUBMITTER: Kundra P 

PROVIDER: S-EPMC10881866 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature