Isolation of circulating endothelial cells provides tool to determine endothelial cell senescence in blood samples.

ABSTRACT: Circulating endothelial cells (CEC) are arising as biomarkers for vascular diseases. However, whether they can be utilized as markers of endothelial cell (EC) senescence in vivo remains unknown. Here, we present a protocol to isolate circulating endothelial cells for a characterization of their senescent signature. Further, we characterize different models of EC senescence induction in vitro and show similar patterns of senescence being upregulated in CECs of aged patients as compared to young volunteers. Replication-(ageing), etoposide-(DNA damage) and angiotensin II-(ROS) induced senescence models showed the expected cell morphology and proliferation-reduction effects. Expression of senescence-associated secretory phenotype markers was specifically upregulated in replication-induced EC s

SUBMITTER: Kalies K 

PROVIDER: S-EPMC10882010 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature