Oriented display of HIV-1 Env trimers by a novel coupling strategy enhances B cell activation and phagocytosis.

ABSTRACT:

Introduction

Conformationally stabilized Env trimers have been developed as antigens for the induction of neutralizing antibodies against HIV-1. However, the non-glycosylated immunodominant base of these soluble antigens may compete with the neutralizing antibody response. This has prompted attempts to couple Env trimers to organic or inorganic nanoparticles with the base facing towards the carrier. Such a site-directed coupling could not only occlude the base of the trimer, but also enhance B cell activation by repetitive display.

Methods

To explore the effect of an ordered display of HIV-1 Env on microspheres on the activation of Env-specific B cells we used Bind&Bite, a novel covalent coupling approach for conformationally sensitive antigens based on heterodimeric coiled-

SUBMITTER: Di Vincenzo R 

PROVIDER: S-EPMC10882061 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature