Probing Blood Plasma Protein Glycosylation with Infrared Spectroscopy.

ABSTRACT: The health state of an individual is closely linked to the glycosylation patterns of his or her blood plasma proteins. However, obtaining this information requires cost- and time-efficient analytical methods. We put forward infrared spectroscopy, which allows label-free analysis of protein glycosylation but so far has only been applied to analysis of individual proteins. Although spectral information does not directly provide the molecular structure of the glycans, it is sensitive to changes therein and covers all types of glycosidic linkages. Combining single-step ion exchange chromatography with infrared spectroscopy, we developed a workflow that enables the separation and analysis of major protein classes in blood plasma. Our results demonstrate that infrared spectroscopy can identify d

SUBMITTER: Voronina L 

PROVIDER: S-EPMC10882574 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature