Chromatographic single-step purification of tagless proteins using gp41-1 split inteins.

ABSTRACT: The current trend in biopharmaceutical drug manufacturing is towards increasing potency and complexity of products such as peptide scaffolds, oligonucleotides and many more. Therefore, a universal affinity purification step is important in order to meet the requirements for cost and time efficient drug production. By using a self-splicing intein affinity tag, a purification template is generated that allows for a universal chromatographic affinity capture step to generate a tagless target protein without the use of proteases for further tag removal. This study describes the successful implementation of gp41-1-based split inteins in a chromatographic purification process for, e.g., E. coli-derived targets. The tagless target is generated in a single-step purification run. The on-colu

SUBMITTER: Knapp M 

PROVIDER: S-EPMC10882715 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature