Multi-species microarrays reveal the effect of sequence divergence on gene expression profiles.

ABSTRACT: Interspecies comparisons of gene expression levels will increase our understanding of the evolution of transcriptional mechanisms and help to identify targets of natural selection. This approach holds particular promise for apes, as many human-specific adaptations are thought to result from differences in gene expression rather than in coding sequence. To date, however, all studies directly comparing interspecies gene expression have been performed on single-species arrays, so that it has been impossible to distinguish differential hybridization due to sequence mismatches from underlying expression differences. To evaluate the severity of this potential problem, we constructed a new multiprimate cDNA array using probes from human, chimpanzee, orangutan, and rhesus. We find a large effect o

SUBMITTER: Gilad Y 

PROVIDER: S-EPMC1088295 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature