Control of asparagine homeostasis in Bacillus subtilis: identification of promiscuous amino acid importers and exporters.

ABSTRACT: The Gram-positive model bacterium B. subtilis is able to import all proteinogenic amino acids from the environment as well as to synthesize them. However, the players involved in the acquisition of asparagine have not yet been identified for this bacterium. In this work, we used d-asparagine as a toxic analog of l-asparagine to identify asparagine transporters. This revealed that d- but not l-asparagine is taken up by the malate/lactate antiporter MleN. Specific strains that are sensitive to the presence of l-asparagine due to the lack of the second messenger cyclic di-AMP or due to the intracellular accumulation of this amino acid were used to isolate and characterize suppressor mutants that were resistant to the presence of otherwise growth-inhibiting concentrations of l-asparagin

SUBMITTER: Meißner J 

PROVIDER: S-EPMC10882977 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature