Detection of Klebsiella pneumoniae in healthy poultry: Insights and perspectives from culturing and metagenomics.

ABSTRACT: Previously, Klebsiella pneumoniae was found to occur more frequently in healthy turkey flocks than in healthy broiler flocks in Norway. This study aimed to investigate whether this higher occurrence could be attributed to a greater abundance of K. pneumoniae in turkey flocks. We compared culturing, qPCR, and shotgun metagenomic sequencing for the detection and quantification of K. pneumoniae. Using qPCR, we found that 20.7% of broiler flock samples and 63.9% of turkey flock samples were positive for K. pneumoniae. Culturing revealed a significantly higher abundance of K. pneumoniae in turkey flocks compared to broiler flocks. However, metagenomic analysis showed no difference in the relative abundance of Klebsiella spp. between broiler and turkey flocks, and no correlation between the resu

SUBMITTER: Kaspersen H 

PROVIDER: S-EPMC10883787 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature