Analysis of quality metrics in comprehensive cancer genomic profiling using a dual DNA-RNA panel.

ABSTRACT:

Background

The nucleic acid quality from formalin-fixed paraffin-embedded (FFPE) tumor vary among samples, resulting in substantial variability in the quality of comprehensive cancer genomic profiling tests. The objective of the study is to investigate how nucleic acid quality affects sequencing quality. We also examined the variations in nucleic acid quality among different hospitals or cancer types.

Methods

Three nucleic acid quality metrics (ddCq, Q-value, and DV200) and five sequencing quality metrics (on-target rate, mean depth, coverage uniformity, target exon coverage, and coverage of the housekeeping gene) were examined using 585 samples from the Todai OncoPanel, a dual DNA-RNA panel.

Results

In the DNA panel, ddCq served as an indicator of sequencing depth an

SUBMITTER: Watanabe K 

PROVIDER: S-EPMC10883814 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature