Detection of feline immunodeficiency virus by neutral red-based loop-mediated isothermal amplification assay.

ABSTRACT:

Background and aim

Feline immunodeficiency virus (FIV) is a retroviral pathogen globally responsible for immunodeficiency disease in cats. However, the current diagnosis based on antibody detection has limitations and can also produce false-positive results. This study aimed to develop a one-pot loop-mediated isothermal amplification (LAMP) process integrated with neutral red (NR-LAMP) assay for detection of FIV proviral DNA.

Materials and methods

We developed a one-pot, gag gene-based NR-LAMP for convenient, rapid, specific, and sensitive colorimetric inspection of FIV proviral DNA.

Results

The developed NR-LAMP was capable of amplifying at an optimum temperature of 65°C for 40 min. No cross-amplification was detected between FIV and other feline viruses teste

SUBMITTER: Saejung W 

PROVIDER: S-EPMC10884571 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature