Deciphering amyloid fibril molecular maturation through FLIM-phasor analysis of thioflavin T.

ABSTRACT: The investigation of amyloid fibril formation is paramount for advancing our understanding of neurodegenerative diseases and for exploring potential correlated therapeutic strategies. Moreover, the self-assembling properties of amyloid fibrils show promise for the development of advanced protein-based biomaterials. Among the methods employed to monitor protein aggregation processes, fluorescence has emerged as a powerful tool. Its exceptional sensitivity enables the detection of early-stage aggregation events that are otherwise challenging to observe. This research underscores the pivotal role of fluorescence analysis, particularly in investigating the aggregation processes of hen egg white lysozyme, a model protein extensively studied for insights into amyloid fibril formation. By combini

SUBMITTER: Anselmo S 

PROVIDER: S-EPMC10884809 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature