Philament: A filament tracking program to quickly and accurately analyze in vitro motility assays.

ABSTRACT: In vitro motility (IVM) assays allow for the examination of the basic interaction between cytoskeletal filaments with molecular motors and the influence many physiological factors have on this interaction. Examples of factors that can be studied include changes in ADP and pH that emulate fatigue, altered phosphorylation that can occur with disease, and mutations within myofilament proteins that cause disease. While IVM assays can be analyzed manually, the main limitation is the ability to extract accurate data rapidly from videos collected without individual bias. While programs have been created in the past to enable data extraction, many are now out of date or require the use of proprietary software. Here, we report the generation of a Python-based tracking program, Philament, which auto

SUBMITTER: Bowser RM 

PROVIDER: S-EPMC10884813 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature