Bacterial surface display of human lectins in Escherichia coli.

ABSTRACT: Lectin-glycan interactions sustain fundamental biological processes involved in development and disease. Owing to their unique sugar-binding properties, lectins have great potential in glycobiology and biomedicine. However, their relatively low affinities and broad specificities pose a significant challenge when used as analytical reagents. New approaches for expression and engineering of lectins are in demand to overcome current limitations. Herein, we report the application of bacterial display for the expression of human galectin-3 and mannose-binding lectin in Escherichia coli. The analysis of the cell surface expression and binding activity of the surface-displayed lectins, including point and deletion mutants, in combination with molecular dynamics simulation, demonstrate the robustn

SUBMITTER: Vazquez-Arias A 

PROVIDER: S-EPMC10884992 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature