Micropreparative Gel Electrophoresis for Purification of Nanoscale Bioconjugates.

ABSTRACT: Conventional techniques for purifying macromolecular conjugates often require complex and costly installments that are inaccessible to most laboratories. In this work, we develop a one-step micropreparative method based on a trilayered polyacrylamide gel electrophoresis (MP-PAGE) setup to purify biological samples, synthetic nanoparticles, as well as biohybrid complexes. We apply this method to recover DNA from a ladder mixture with yields of up to 90%, compared to the 58% yield obtained using the conventional crush-and-soak method. MP-PAGE was also able to isolate enhanced yellow fluorescence protein (EYFP) from crude cell extract with 90% purity, which is comparable to purities achieved through a more complex two-step purification procedure involving size exclusion and immobilized metal-

SUBMITTER: Sajjadi SH 

PROVIDER: S-EPMC10885001 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature