Interfacing Aptamer-Modified Nanopipettes with Neuronal Media and Ex Vivo Brain Tissue.

ABSTRACT: Aptamer-functionalized biosensors exhibit high selectivity for monitoring neurotransmitters in complex environments. We translated nanoscale aptamer-modified nanopipette sensors to detect endogenous dopamine release in vitro and ex vivo. These sensors employ quartz nanopipettes with nanoscale pores (ca. 10 nm diameter) that are functionalized with aptamers that enable the selective capture of dopamine through target-specific conformational changes. The dynamic behavior of aptamer structures upon dopamine binding leads to the rearrangement of surface charge within the nanopore, resulting in measurable changes in ionic current. To assess sensor performance in real time, we designed a fluidic platform to characterize the temporal dynamics of nanopipette sensors. We then conducte

SUBMITTER: Stuber A 

PROVIDER: S-EPMC10885324 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature