Electrochemical Visualization of Single-Molecule Thiol Substitution with Nanopore Measurement.

ABSTRACT: Reactions involving sulfhydryl groups play a critical role in maintaining the structure and function of proteins. However, traditional mechanistic studies have mainly focused on reaction rates and the efficiency in bulk solutions. Herein, we have designed a cysteine-mutated nanopore as a biological protein nanoreactor for electrochemical visualization of the thiol substitute reaction. Statistical analysis of characteristic current signals shows that the apparent reaction rate at the single-molecule level in this confined nanoreactor reached 1400 times higher than that observed in bulk solution. This substantial acceleration of thiol substitution reactions within the nanopore offers promising opportunities for advancing the design and optimization of micro/nanoreactors. Moreover, our result

SUBMITTER: Yang CN 

PROVIDER: S-EPMC10885329 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature