HILIC-IM-MS for Simultaneous Lipid and Metabolite Profiling of Bacteria.

ABSTRACT: Although MALDI-ToF platforms for microbial identifications have found great success in clinical microbiology, the sole use of protein fingerprints for the discrimination of closely related species, strain-level identifications, and detection of antimicrobial resistance remains a challenge for the technology. Several alternative mass spectrometry-based methods have been proposed to address the shortcomings of the protein-centric approach, including MALDI-ToF methods for fatty acid/lipid profiling and LC-MS profiling of metabolites. However, the molecular diversity of microbial pathogens suggests that no single "ome" will be sufficient for the accurate and sensitive identification of strain- and susceptibility-level profiling of bacteria. Here, we describe the development of an alternative a

SUBMITTER: Carpenter JM 

PROVIDER: S-EPMC10885331 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature