Microbial-enrichment method enables high-throughput metagenomic characterization from host-rich samples.

ABSTRACT: Host-microbe interactions have been linked to health and disease states through the use of microbial taxonomic profiling, mostly via 16S ribosomal RNA gene sequencing. However, many mechanistic insights remain elusive, in part because studying the genomes of microbes associated with mammalian tissue is difficult due to the high ratio of host to microbial DNA in such samples. Here we describe a microbial-enrichment method (MEM), which we demonstrate on a wide range of sample types, including saliva, stool, intestinal scrapings, and intestinal mucosal biopsies. MEM enabled high-throughput characterization of microbial metagenomes from human intestinal biopsies by reducing host DNA more than 1,000-fold with minimal microbial community changes (roughly 90% of taxa had no significant difference

SUBMITTER: Wu-Woods NJ 

PROVIDER: S-EPMC10885704 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature