Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid.

ABSTRACT: A liquid chromatography tandem mass spectrometry method was developed for quantifying ten cannabinoids in oral fluid (OF). This method utilizes OF collected by the Quantisal™ device and concurrently quantifies cannabinol (CBN), cannabidiol (CBD), Δ⁹-tetrahydrocannabinol (THC), 11-hydroxy-Δ⁹-THC (11-OH-THC), 11-nor-9-carboxy-Δ⁹-THC (THC-COOH), 11-nor-9-carboxy-Δ⁹-THC glucuronide (THC-COOH-gluc), Δ⁹-THC glucuronide (THC-gluc), cannabigerol (CBG), tetrahydrocannabiverin (THCV), and Δ⁹-tetrahydrocannabinolic acid A (THCA-A). Solid phase extraction was optimized using Oasis Prime HLB 30 mg 96-well plates. Cannabinoids were separated by liquid chromatography over a BEH C18 column and detected by a Waters TQ-S micro tandem mass spe

SUBMITTER: Sobolesky PM 

PROVIDER: S-EPMC10885733 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature