A Kinetic Approach to Oxygen Radical Absorbance Capacity (ORAC): Restoring Order to the Antioxidant Activity of Hydroxycinnamic Acids and Fruit Juices.

ABSTRACT: This study introduces a kinetic model that significantly improves the interpretation of the oxygen radical absorbance capacity (ORAC) assay. Our model accurately simulates and fits the bleaching kinetics of fluorescein in the presence of various antioxidants, achieving high correlation values (R² > 0.99) with the experimental data. The fit to the experimental data is achieved by optimizing two rate constants, k₅ and k₆. The k₅ value reflects the reactivity of antioxidants toward scavenging peroxyl radicals, whereas k₆ measures the ability of antioxidants to regenerate oxidized fluorescein. These parameters (1) allow the detailed classification of cinnamic acids based on their structure-activity relationships

SUBMITTER: Asma U 

PROVIDER: S-EPMC10886186 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature