NOTCH1 as a Negative Regulator of Avian Adipocyte Differentiation: Implications for Fat Deposition.

ABSTRACT: The NOTCH signaling pathway plays a pivotal role in diverse developmental processes, including cell proliferation and differentiation. In this study, we investigated whether this signaling molecules also contribute to avian adipogenesis. Using previous mRNA-seq datasets, we examined the expression of 11 signaling members during avian adipocyte differentiation. We found most members are down-regulated throughout differentiation (p < 0.05). As a representative, NOTCH1 was decreased in cultured chicken abdominal adipocytes during adipogenesis at mRNA and protein levels (p < 0.05). Moreover, using an overexpression plasmid for NOTCH1's intracellular domain (NICD1), as well as siRNA and DAPT to activate or deplete NOTCH1 in cells, we investigated the role of NOTCH

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10886207 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature