An Efficient Homologous Recombination-Based In Situ Protein-Labeling Method in Verticillium dahliae.

ABSTRACT: Accurate determination of protein localization, levels, or protein-protein interactions is pivotal for the study of their function, and in situ protein labeling via homologous recombination has emerged as a critical tool in many organisms. While this approach has been refined in various model fungi, the study of protein function in most plant pathogens has predominantly relied on ex situ or overexpression manipulations. To dissect the molecular mechanisms of development and infection for Verticillium dahliae, a formidable plant pathogen responsible for vascular wilt diseases, we have established a robust, homologous recombination-based in situ protein labeling strategy in this organism. Utilizing Agrobacterium tumefaciens-mediated transformation (ATMT), this methodology facil

SUBMITTER: Yang J 

PROVIDER: S-EPMC10886240 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature