Characterization of Novel RHD Allele Variants and Their Implications for Routine Blood Group Diagnostics.

ABSTRACT: The Rh system, including the highly immunogenic D antigen, is one of the clinically most important blood group systems in transfusion medicine. Numerous alleles of the RHD gene are associated with variant RhD phenotypes. In case of Rh incompatibility, some of them can induce hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. Thus, accurate blood group diagnostics are critical for safe transfusion therapy. We characterized phenotypes of four individuals revealing weakened D expression during routine pre-transfusion testing. Standard gel card matrix techniques with monoclonal and polyclonal anti-D antibodies were used for serological typing, complemented using D epitope and antigen density analysis. Genotyping employing PCR with sequence-specific primers,

SUBMITTER: Matzhold EM 

PROVIDER: S-EPMC10886452 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature