Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices.

ABSTRACT: Amperometry is arguably the most widely used technique for studying the exocytosis of biological amines. However, the scarcity of human tissues, particularly in the context of neurological diseases, poses a challenge for exocytosis research. Human platelets, which accumulate 90% of blood serotonin, release it through exocytosis. Nevertheless, single-cell amperometry with encapsulated carbon fibers is impractical due to the small size of platelets and the limited number of secretory granules on each platelet. The recent technological improvements in amperometric multi-electrode array (MEA) devices allow simultaneous recordings from several high-performance electrodes. In this paper, we present a comparison of three MEA boron-doped diamond (BDD) devices for studying serotonin exocytosis in h

SUBMITTER: Gonzalez Brito R 

PROVIDER: S-EPMC10886747 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature