Morphological Changes of 3T3 Cells under Simulated Microgravity.

ABSTRACT:

Background

Cells are sensitive to changes in gravity, especially the cytoskeletal structures that determine cell morphology. The aim of this study was to assess the effects of simulated microgravity (SMG) on 3T3 cell morphology, as demonstrated by a characterization of the morphology of cells and nuclei, alterations of microfilaments and microtubules, and changes in cycle progression.

Methods

3T3 cells underwent induced SMG for 72 h with Gravite^®, while the control group was under 1G. Fluorescent staining was applied to estimate the morphology of cells and nuclei and the cytoskeleton distribution of 3T3 cells. Cell cycle progression was assessed by using the cell cycle app of the Cytell microscope, and Western blot was conducted to determine the expression of the

SUBMITTER: Tran MT 

PROVIDER: S-EPMC10887114 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature