Electrochemical Aptasensing Platform for the Detection of Retinol Binding Protein-4.

ABSTRACT: Here, we present the results of our the electrochemical aptasensing strategy for retinol binding protein-4 (RBP-4) detection based on a thiolated aptamer against RBP-4 and 6-mercaptohexanol (MCH) directly immobilized on a gold electrode surface. The most important parameters affecting the magnitude of the analytical signal generated were optimized: (i) the presence of magnesium ions in the immobilization and measurement buffer, (ii) the concentration of aptamer in the immobilization solution and (iii) its folding procedure. In this work, a systematic assessment of the electrochemical parameters related to the optimization of the sensing layer of the aptasensor was carried out (electron transfer coefficients (α), electron transfer rate constants (k⁰) and surface cov

SUBMITTER: Malecka-Baturo K 

PROVIDER: S-EPMC10887324 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature