The Impact of 3D Nichoids and Matrix Stiffness on Primary Malignant Mesothelioma Cells.

ABSTRACT: Malignant mesothelioma is a type of cancer that affects the mesothelium. It is an aggressive and deadly form of cancer that is often caused by exposure to asbestos. At the molecular level, it is characterized by a low number of genetic mutations and high heterogeneity among patients. In this work, we analyzed the plasticity of gene expression of primary mesothelial cancer cells by comparing their properties on 2D versus 3D surfaces. First, we derived from primary human samples four independent primary cancer cells. Then, we used Nichoids, which are micro-engineered 3D substrates, as three-dimensional structures. Nichoids limit the dimension of adhering cells during expansion by counteracting cell migration between adjacent units of a substrate with their microarchitecture. Tumor cells grow

SUBMITTER: Oliveto S 

PROVIDER: S-EPMC10887956 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature