A Simple, Fast, Sensitive LC-MS/MS Method to Quantify NAD(H) in Biological Samples: Plasma NAD(H) Measurement to Monitor Brain Pathophysiology.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD) is a cofactor in redox reactions and an essential mediator of energy metabolism. The redox balance between NAD⁺ and NADH affects various diseases, cell differentiation, and aging, and in recent years there has been a growing need for measurement techniques with improved accuracy. However, NAD(H) measurements, representing both NAD⁺ and NADH, have been limited by the compound's properties. We achieved highly sensitive simultaneous measurement of NAD⁺ and NADH under non-ion pairing, mobile phase conditions of water, or methanol containing 5 mM ammonium acetate. These were achieved using a simple pre-treatment and 7-min analysis time. Use of the stable isotope ¹³C₅-NAD⁺ as an internal sta

SUBMITTER: Ishima T 

PROVIDER: S-EPMC10888655 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature