Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.

ABSTRACT: Single-molecule super-resolution imaging is instrumental for investigating cellular architecture and organization at the nanoscale. Achieving precise 3D nanometric localization when imaging structures throughout mammalian cells, which can be multiple microns thick, requires careful selection of the illumination scheme in order to optimize the fluorescence signal to background ratio (SBR). Thus, an optical platform that combines different wide-field illumination schemes for target-specific SBR optimization would facilitate more precise, 3D nanoscale studies of a wide range of cellular structures. Here we demonstrate a versatile multimodal illumination platform that integrates the sectioning and background reduction capabilities of light sheet illumination with homogeneous, flat-field epi-an

SUBMITTER: Nelson T 

PROVIDER: S-EPMC10888752 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature