DNA methylation-dependent and -independent binding of CDX2 directs activation of distinct developmental and homeostatic genes.

ABSTRACT: Precise spatiotemporal and cell type-specific gene expression is essential for proper tissue development and function. Transcription factors (TFs) guide this process by binding to developmental stage-specific targets and establishing an appropriate enhancer landscape. In turn, DNA and chromatin modifications direct the genomic binding of TFs. However, how TFs navigate various chromatin features and selectively bind a small portion of the millions of possible genomic target loci is still not well understood. Here we show that Cdx2 - a pioneer TF that binds distinct targets in developing versus adult intestinal epithelial cells - has a preferential affinity for a non-canonical CpG-containing motif in vivo. A higher frequency of this motif at embryonic and fetal Cdx2 target loci and th

SUBMITTER: Lorzadeh A 

PROVIDER: S-EPMC10888781 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature